Figure 4.

Allelic replacement of skaAE395 K provides resistance to inhibitory compounds in Pseudomonas supernatants. We recreated the skaAE395 K allele in a naive chromosomal and megaplasmid background (strain DBL494). A zone of inhibition can be seen for strain DBL494 but not for strain DBL1802. Strain DBL494 contains a wild-type allele of skaA on the megaplasmid while the skaAE395 K mutation has been recombined into the megaplasmid in strain DBL1802. Overlays were plated after 5 h of growth in KB liquid and spotted with 20 µl of P. stutzeri filter-sterilized supernatants. All images are representative of three biological replicates for these strains. Raw image files can be found in electronic supplementary material at Figshare (doi:10.6084/m9.figshare.15072537.v3): DBL494 is electronic supplementary material, figure S8, DBL1802 is electronic supplementary material, figure S10.