Figure 2.

Strains expressing B310 show the toxin phenotype. (a) Uninfected RJW004 strain was transformed with plasmids that contained either both B310 and C108, B310 alone, the originally annotated B252 alone, C108 alone or empty plasmid under the control of inducible arabinose promotor. (b) Growth curve showing that there is a cost to carrying plasmids with the B310 gene. Strains were grown in induced conditions in the presence of arabinose as the carbon source in the medium. Error bars show mean results ± standard error of the means (s.e.m.) (n = 3). (c) Supernatants from RJW004 strains with a plasmid encoding B310 can produce a zone of inhibition on uninfected strain RJW002. (d) Supernatants from B310 overexpression strain or boiled supernatant from the chronically SSV9-infected strain were spotted onto lawns of the RJW004 strain carrying pOE-B310, pOE-B252, pOE-C108 and pOE-Empty. A plus sign indicates the presence of a zone of inhibition and a negative sign indicates the absence of a zone of inhibition.