Figure 4.

Examination of killing phenotype of putative toxins derived from SSV11 (a) and SSV13 (b). Uninfected strain RJW004 was transformed with Sulfolobus expression plasmid carrying both genes and each gene separately. Ten microlitres of supernatants from overexpression strains under inducing conditions was spotted on lawns of a tester strain RJW002, and then incubated at 76–78°C. Plates were imaged after 3–4 days of incubation. At least three biological replicates were set up for each sample.