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. 2021 Nov 29;377(1842):20200476. doi: 10.1098/rstb.2020.0476

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© 2021 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Cross-challenge of chronically infected strains among SSV toxins. (a) Schematic showing the lawns of different host and supernatant of RJW004 strains harbouring different plasmid constructs that were used in the spot-on-lawn assay. (b) Spot-on-lawn assay. Ten microlitres of collected supernatants and medium control was spotted on lawns of uninfected host (RJW002 and Δcas6) and SSV-infected strains. Plates were imaged after 3–4 days of incubation at 76–78°C. The plate image shown here is a representative from three biological replicates.