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Elution profile of EMCP on DEAE-cellulose and gel-permeation chromatography. (A) EMCP was applied to a DEAE-cellulose column, and five fractions of EMCP were eluted, initially using dH2O (EMCP-N), followed by NaCl(aq) at different concentrations of 0.05 M (EMCP-1), 0.1 M (EMCP-2), 0.2 M (EMCP-3), and 0.3 M (EMCP-4). (B) EMCP-1, EMCP-2, and EMCP-3 were applied to a Sephadex G-75 column, and EMCP-4 was applied to a Sepharose CL-6B column.
