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. 2021 Nov 26;219(1):e20210789. doi: 10.1084/jem.20210789

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© 2021 Guo et al.

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Figure 4. — FOXM1 binds to the SETDB1 promoter regulating ERVs transcription. (A) Protein lysates from indicated cells treated with AZD1775 were analyzed by RPPA (n = 2). Heatmap of RPPA data representing “rank-ordered” changes induced by AZD1775. (B) Enrichr analysis of transcription factors associated with genes down-regulated in AZD1775-treated ID8. (C) RPPA profiling in OVCAR4 cells after AZD1775 or FOXM1 silencing with siRNA (n = 2). (D) Western blot of indicated proteins in OVCAR4 cells treated as indicated. The data represent three independent experiments. (E) Heatmap of differentially expressed genes detected by NanoString immune panel in parental or ectopic FOXM1 expressing OVCAR4 cells with or without AZD1775 treatment (n = 3). (F) Screenshot of assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq), H3K4me3, H3K27me3, and FOXM1 ChIP-seq tracks of SETDB1 in MDA-MB-231 cells. (G) ChIP-qPCR analysis of FOXM1 binding in the SETDB1 promoter region with or without AZD1775 treatment in OV90 cells (n = 3; three independent experiments). Data across panels were mean ± SEM. **, P < 0.01, as determined by unpaired t test.