Figure 3.

Effect of CMTM5 on DU145 cells. (A) Western blot analysis of relative CMTM5 protein expression in CMTM5-overexpressing cells and vector control cells. ***P<0.001. (B) Cell Counting Kit-8 assay results of the proliferation of CMTM5-overexpressing cells and vector control cells. *P<0.05 vs vector control cells. (C) Colony-forming capacity was measured by counting number of colonies with ≥50 cells. ***P<0.001. (D) Wound healing assay of DU145 cells transfected with overexpressing CMTM5 lentivirus vector. ***P<0.001. Scale bar, 100 µm. (E) Transwell migration and invasion assays were performed to evaluate the impact of CMTM5 on the migration and invasion of PCa cells. Scale bar, 20 µm. ***P<0.001. Values are expressed as the mean ± SD of the mean and are representative of three independent experiments. PCa, prostate cancer; CMTM5, CKLF-like Marvel transmembrane domain containing family member 5; OD, optical density.