Normal growth and liver functions of C/ebpαβ/β mice. (A) Growth curves for C/ebpαβ/β mice on a standard diet. The value for each point is the average body weight of five mice of the same genotype. The standard deviation for each group is within 10% of the respective mean value and is not shown. (B) Histological sections and H&E staining of livers of 9-week-old C/ebpαβ/β mice. Magnification, ×360 (original magnification, ×400). (C) Northern blotting analysis of representative liver biopsies of C/ebpαβ/β mice. Liver total RNAs (20 μg) from C/ebpαβ/β mice of different ages were denatured and electrophoresed on a formaldehyde-containing 1% agarose gel, blotted to a nylon membrane, and probed with the indicated cDNA probes. Each lane contains RNA from an individual animal. E, embryonic stage; NB, newborn; W, week. (D) Western analysis of C/EBPβ protein levels of representative liver and WAT biopsies of C/ebpαβ/β mice. Total cellular protein (50 μg) from livers from mice of different ages and from WAT of 7-week-old mice were separated on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, blotted to a nitrocellulose membrane, and probed with an antibody against mouse C/EBPβ. Each lane contains protein from an individual animal. E, embryonic stage; W, week. (E) Northern blotting analysis of representative liver biopsies of C/ebpαβ/β mice after the LPS treatment. Liver total RNA (20 μg) from 4-week-old C/ebpαβ/β mice injected with LPS were denatured and electrophoresed on a formaldehyde-containing 1% agarose gel, blotted to a nylon membrane, and probed with the indicated oligonucleotide probes. Each lane contains RNA from an individual animal. +, wild-type allele; β, targeted C/ebpα allele.