Decreased expressions of transferrin and CDO genes in WAT, but not in liver, of C/ebpαβ/β mice. Northern blotting analysis of representative tissue biopsies of C/ebpαβ/β mice. Total RNA isolated from tissues of C/ebpαβ/β mice were denatured and electrophoresed on a formaldehyde-containing 1% agarose gel, blotted to a nylon membrane, and probed with the indicated cDNA probes. Each lane contains RNA from an individual animal. +, wild-type allele; β, targeted C/ebpα allele.