Figure 1.

Reversal of splenic phenotypes in ABIN1[D485N] knock-in mice by an IRAK4 inhibitor. 13-week-old ABIN1[D485N] mice (n=6) and age-matched wild-type (WT) (n=5) mice were fed for 10 weeks on R&M3 diet containing or not containing PF 06426779 (4 g/kg). Mice were culled at the age of 23 weeks. An additional group of 8 WT and 6 ABIN1[D485N] knock-in mice were culled when they were 13 weeks old as a control. (A) Spleen weight and a representative image showing relative spleen size. Bar equals 1 cm. (B) Neutrophil numbers in the spleen. (C) Germinal Centre B cell numbers in the spleen. (D) Follicular T helper cell numbers in the spleen. Each symbol shows the result from a single individual mouse. Statistical significance was calculated using one-way ANOVA and Tukey’s post hoc test (A, B and D) or the Kruskal-Wallis and the Mann-Whitney U tests (C); * denotes p<0.05, ** denotes p<0.01 and *** denotes p<0.001. The methodology used is detailed in Nanda et al.⁶