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. Author manuscript; available in PMC: 2021 Nov 29.
Published in final edited form as: Nat Genet. 2021 May 6;53(6):869–880. doi: 10.1038/s41588-021-00861-8

Extended Data Fig. 3. ABE mutagenesis at different genomic loci.

Extended Data Fig. 3

(a) Bar plot showing the effects of NFIX CREs on the expression levels of NFIX and KLF1. Y-axis shows relative mRNA expression measured by real-time RT-qPCR in HUDEP-2 cells edited with ABEmax and the two indicated gRNAs. The expression levels were normalized by those from HUDEP-2 cells treated with ABEmax and non-targeting control gRNA (Ctrl) (n=3 independent experiments).

(b) β-Like globin gene cluster–associated HbFhigh gRNAs: Chromatin interaction loops, indicated by red arcs, were determined by H3K27ac Hi-ChIP in HUDEP-2 cells. gRNA -log(FDR) represents the difference in gRNA abundance between the HbFhigh and HbFlow populations. ATAC-seq analysis reflects chromatin openness.