KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD3-APC (clone 17A2) | eBioscience | Cat# 12-0032-82 |
| CD69-PE (clone H1.2F3) | eBioscience | Cat# 12-0691-83 |
| CD69-PE-CF594 (clone H1.2F3) | BD Horizon | Cat# 562455 |
| CXCR3-BV421 (clone 173) | Biolegend | Cat# 126522 |
| Ly-6G-FITC(Clone 1A8) | Biolegend | Cat# 127605 |
| TER-119-APC/Cy7 (Clone TER-119) | Biolegend | Cat# 116223 |
| CD62L-PE/Dazzle (clone MEL-14) | Biolegend | Cat# 104448 |
| CD62L-PerCP-Cy5.5 (clone MEL-14) | Biolegend | Cat #104432 |
| CD62L-APC (clone MEL-14) | BD | Cat# 553152 |
| CD62L-FITC (clone MEL-14) | eBioscience | Cat #11-0621-85 |
| MHC-II (I-A/I-E)-BV510 (clone M5/114.15.2) | Biolegend | Cat# 107635 |
| CX3CR1-PE/Cy7 (clone SA011F11) | Biolegend | Cat# 149016 |
| Ly6C-FITC (clone AL-21) | BD Biosciences | Cat# 553104 |
| Ly-6C-PE/Cy7 (Clone AL-21) | BD | 560593 |
| F4/80-PE (clone BM8) | Biolegend | Cat# 123110 |
| F4/80-AF647 (clone BM8) | Biolegend | Cat# 123122 |
| F4/80-BV421 (clone BM8) | Biolegend | Cat# 123137 |
| CD45.2-vF450 (clone 104) | Tonbo | Cat# 75-0454-U100 |
| CXCR6-PE (Clone SA051D1) | Biolegend | Cat# 151104 |
| CD64-BV786 (Clone X54-5/7.1) | BD | Cat# 741024 |
| CD11b-PE (Clone M1/70) | Tonbo | Cat# 50-0112-U100 |
| CD8α-BV785 (Clone 53-6.7) | Biolegend | Cat# 100750 |
| CD8α-PercpCy5.5 (clone 53-6.7) | BioLegend | Cat# 100734 |
| CD11a-BV510 (clone M17/4) | BD Horizon | Cat# 624144 |
| CD90.1-PE (clone OX-7) | BioLegend | Cat# 202524 |
| CD90.1-APC (clone OX-7) | eBioscience | Cat# 17-0900-82 |
| CD45.1-FITC (clone A20) | BD | Cat# 553775 |
| CD45.1-ef450 (clone A20) | Invitrogen | Cat# 48-0453-82 |
| CD49b-PE-Cy7 (clone DX5) | eBioscience | Cat# 25-5971-81 |
| CD42d-APC (clone 1C2) | Biolegend | Cat# 148505 |
| CD11c-APC (clone N418) | Biolegend | Cat# 117310 |
| B220-APC (clone RA3-6B2) | Tonbo | Cat# 20-0452-U100 |
| CD49a-BB700 (clone Ha31/8) | BD Horizon | Cat# 742164 |
| CD54-BV421 (clone YN1/1.7.4) | Biolegend | Cat# 116141 |
| CD43-PE/Dazzle 594 (clone 1B11) | Biolegend | Cat# 121226 |
| Ki67-BV421 | BD | Cat# 562899 |
| Anti-mouse LFA-1a (clone M17/4) | BioXcell | Cat# BE0006 |
| Anti-mouse CXCR3 (clone 173) | BioXcell | Cat# BE0249 |
| Anti-mouse/human CD44 (clone IM7) | BioXcell | Cat# BE0039 |
| Anti-mouse CD41 (clone MWReg30) | Biolegend | Cat# 133902 |
| Anti-mouse IFNγ (clone XMG1.2) | Prepared in house | N/A |
| Anti-mouse NK1.1 (clone PK136) | Prepared in house | N/A |
| Mouse IgG2a isotype control (clone C1.18.4) | BioXcell | Cat# BE0085 |
| Anti-horseradish peroxidase (clone HRPN) | BioXcell | Cat# BE0088 |
| Armenian hamster IgG isotype control (polyclonal) | BioXcell | Cat# BE0091 |
| H-2Db-GAP5040-48 | Prepared in house | N/A |
| H-2Kb-OVA257-264 | Prepared in house | N/A |
| H-2Db-CS252-260 | Prepared in house | N/A |
| 24.2G Fc block | Prepared in house | N/A |
| Bacterial and virus strains | ||
| P. berghei ANKA parasitized-Anopheles stephensi | Harty lab insectary | N/A |
| P. berghei ANKA-sOva parasitized-Anopheles stephensi | Harty lab insectary | N/A |
| P. berghei ANKA GFP-parasitized Anopheles stephensi | NYU Langone Health Insectary Core & Parasite Culture | N/A |
| P. berghei ANKA luciferase-parasitized Anopheles stephensi | SporoCore, University of Georgia, Athens, GA | N/A |
| Listeria monocytogenes expressing Pb-CS252, attenuated (ΔactA, ΔInlB-deficient) | Harty lab | N/A |
| Listeria monocytogenes expressing Pb-GAP5041, attenuated (ΔactA, ΔInlB-deficient) | Harty lab | N/A |
| Listeria monocytogenes expressing OVA257-264, attenuated (ΔactA, ΔInlB-deficient) | Harty lab | N/A |
| Listeria monocytogenes expressing LCMV-GP33, attenuated (ΔactA, ΔInlB-deficient) | Harty lab | N/A |
| Listeria monocytogenes expressing OVA257-264, virulent | Harty lab | N/A |
| Recombinant influenza PR8-OVA virus | Harty lab | N/A |
| X31 influenza virus | Harty lab | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| RNAprotect® Tissue Reagent | QIAGEN | Cat# 76106 |
| Liver Digest Medium | Thermo Fisher Scientific | Cat# 17703034 |
| LPS | Sigma | Cat# L-8274 |
| Brefeldin A (1,000x) | BioLegend | Cat# 420601 |
| Collagenase Type II | GIBCO | Cat# 17101-015 |
| DNase | Sigma-Aldrich | Cat# D4513-1VL |
| DPBS | GIBCO | Cat# 14190-144 |
| DMEM | GIBCO | Cat# 11965-092 |
| HBSS | GIBCO | Cat# 14025-092 |
| RPMI | GIBCO | Cat# 11875-093 |
| Percoll | GE Healthcare | Cat# 17-0891-01 |
| Vitalyse | CMDG | Cat# WBL0100 |
| Fixable Viability Stain | BD Horizon | Cat# 565388 |
| Cytofix Fixation Buffer | BD Bioscience | Cat# 554655 |
| Cell Staining Buffer | Biolegend | Cat# 420201 |
| TRIzol | Ambion | Cat# 1559601 |
| Propidium iodide | Invitrogen | Cat# V35118 |
| Mouse CD1 serum | Innovative Research | Cat# IGMSCD1SER10ML-36003 |
| Critical commercial assays | ||
| Anti-CD11c MicroBeads UltraPure mouse | Miltenyi Biotec | Cat# 130-108-338 |
| CD8α+ T Cell Isolation Kit mouse | Miltenyi Biotec | Cat# 130-104-075 |
| LS Columns | Miltenyi Biotec | Cat# 130-042-401 |
| RNeasy Plus Mini Kit (RNA sequencing) | QIAGEN | Cat# 74134 |
| QIAshredder (RNA sequencing) | QIAGEN | Cat# 79654 |
| RNA Clean & Concentrator −25 (RT-qPCR) | Zymo Research | Cat# R1018 |
| Deposited data | ||
| RNA Sequencing (Figure 4) | https://www.ncbi.nlm.nih.gov/geo/ | GSE178821 |
| Experimental models: cell lines | ||
| B16-Flt-3L cell line | Mach et al., 2000 | B16-FLT3L (RRID:CVCL_IJ12) |
| Experimental models: organisms/strains | ||
| Mouse: CB6F1/Crl | Harty Laboratory and National Cancer Institute | #176 |
| Mouse: C57BL/6J | Harty Laboratory and National Cancer Institute | #556 |
| Mouse: C57BL/6J | Harty Laboratory and The Jackson Laboratory | #000664 |
| Mouse: CB6F1 with CS252-260- specific TCRαβ chain retrogenic | This manuscript | N/A |
| Mouse: CB6F1 with OVA257-264- specific TCRαβ chain retrogenic | This manuscript | N/A |
| Mouse: B6.129S7-Itgaltm1Bll/J (LFA-1 KO) | The Jackson Laboratory | #005257 |
| Mouse: C57BU6-Gt(ROSA)26Sortm1(HBEGF)Awai/J (ROSA26iDTR) | The Jackson Laboratory | #007900 |
| Mouse: C57BL/6J-Clec4fem1(cre)Glass/J (CLec4f-Cre-tdTomato) | The Jackson Laboratory | #033296 |
| Mouse: B6.FVB-1700016L21RikTg(Itgax-DTR/EGFP)57Lan/J (CD11c-DTR) | Harty Laboratory and The Jackson Laboratory | #004509 |
| Mouse: C57BL/6-Tg(Tcracrb)1100Mjb/J (OT-1) | Harty Laboratory and The Jackson Laboratory | #003831 |
| Mouse: B6.Cg-Tcratm1Mom Tg(TcrLCMV)327Sdz/TacMmjax (P14 TCRVα2Vβ8) | Harty Laboratory and The Jackson Laboratory | #37394-JAX |
| Software and algorithms | ||
| FlowJo 10.7.1 | FlowJo (BD) | https://www.fiowjo.com/solutions/flowjo/downloads |
| GraphPad Prism 9.1.1 (223) | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Adobe Illustrator 25.2.3 | Adobe | https://www.adobe.com/products/illustrator.html |
| IMGT | IMGT®, the international ImMunoGeneTics information system | http://www.imgt.org |
| RStudio 3.6.1 | RStudio | https://www.rstudio.com/ |
| edgeR 3.4.0 | Bioconductor | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| DSeq2 1.26.0 | Bioconductor | https://bioconductor.org/packages/3.13/bioc/html/DESeq2.html |
| GSEA desktop application 4.1.0 [build 27] | Gene Set Enrichment Analysis (UC San Diego) | https://www.gsea-msigdb.org/gsea/index.jsp |
| Trim Galore 0.6.5 | Babraham Bioinformatics | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ |
| Kallisto | Patcher lab | https://pachterlab.github.io/kallisto/ |
| Leica X 3.1.5.16308 | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| Imaris x64 9.7.1 | Imaris (Oxford Instruments Group) | https://imaris.oxinst.com/ |