. 2021 Nov 29;22:72. doi: 10.1186/s40360-021-00540-8

Table 1.

Summary of PCR primer pairs

Name	Sequence of primers	Annealing Temperature
Bax	Forward: 5’CAAGGCCCTGTGCACTAAAGT3’	60 °C
Bax	Reverse: 5’AAGTAGGAGAGGAGGCCTTCC3’	60 °C
Bcl2	Forward: 5’GGAGAAATCAAACAGAGGTCGC3’	60 °C
Bcl2	Reverse: 5’CGTCAACAGGGAGATGTCACC3’	60 °C
MyD88	Forward: 5’CTCCAGGTGTCCAACAGAAGC3’	60 °C
MyD88	Reverse: 5’TCATCTTCCCCTCTGCCCTAG3’	60 °C
NF-κB	Forward: 5’GCCATTGAAGTGATCCAGGCA3’	60 °C
NF-κB	Reverse: 5’TCCCGGAGTTCATCTATGTGCT3’	60 °C
TLR4	Forward 5’GCATGGATCAGAAAACTCAGC −5’	60 °C
TLR4	Reverse: 5’TGTTTCAATTTCACACCTGGA −5’	60 °C
Caspase 3	Forward: 5’GGAGCAGCTTTGTGTGTGTG3’	60 °C
Caspase 3	Reverse: 5’TCCAGGAATAGTAACCAGGTGC3’	60 °C
GAPDH	Forward: 5’GGAAGGTGAAGGTCGGAGTCA3’	60 °C
GAPDH	Reverse: 5’GTCATTGATGGCAACAATATCCACT3’	60 °C