Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pyruvate stimulates an increase in cytosolic JNK1 activity. (A) Time course of JNK1 activity in HeLa cells following pyruvate (100 mM) stimulation. Activity was assessed in 30 μg of protein extract by immunocomplex kinase assays using GST-ATF2 as a substrate. Lysates were also probed by Western blot analysis for JNK1 protein levels. (B) Levels of JNK1 activity 1 h after pyruvate addition in various cell types. The normal concentration of pyruvate in basal medium was 1 mM.
