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Pyruvate addition stimulates the release of mitochondrial oxidants. Confocal image of DHR123 fluorescence (A) under basal conditions and (B) 2 h following 100 mM pyruvate addition. Dose-response of (C) pyruvate-induced DHR123 fluorescence and (D) DCFDA fluorescence. (E) Quantitation of DHR123 fluorescence under basal conditions and 2 h following pyruvate addition in the presence (25 μM for 1 h) and absence of rotenone.
