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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Bone. 2020 Nov 17;143:115762. doi: 10.1016/j.bone.2020.115762

Figure 6: Restoration of increased macrophage numbers and enhanced TGF-β signaling with injected MCP-1 in MCP-1-deficient mice.

Figure 6:

(A) Immunohistochemistry of phospho-Smad2 in the tibiae after 6 weeks of hPTH (1–34; 80 ug/kg) and/or rhMCP-1 (0.25μg/mouse) injections to wild-type and MCP-1−/− mice. rhMCP-1 injections rescued the p-Smad2 staining in MCP-1−/− mice to comparable to hPTH-injected wild-type mice by quantitation of stained cells. (B) Mac3 immunostaining of sections of tibiae of WT and MCP-1−/− mice after 6 weeks of the various treatments. There is a modest expression of Mac-3 in MCP-1 null mice, with and without hPTH injections and rhMCP-1 injections increased the Mac-3 positive cells in the tibiae of MCP-1−/− mice, as seen by the quantitation of stained cells. IgG served as a negative control. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to vehicle-injected WT mice and #p < 0.05 compared to PTH-injected WT mice by two-way ANOVA.