FIG. 4.

Tissue distribution of human KKLF. (a) Northern analysis of human KKLF. Each lane contains polyA(+) RNAs (2 μg) from various human tissues. Lanes: 1, brain; 2, heart; 3, skeletal muscle; 4, colon; 5, thymus; 6, spleen; 7, kidney; 8, liver; 9, small intestine; 10, placenta; 11, lung; 12, leukocyte. (b) Relative KKLF expression. A dot blot analysis of 0.5 μg of poly(A)⁺ RNA was performed using the human RNA Master Blot (Clontech). Hybridization signals were quantified using an image analyzer (BAS-2500; Fuji Corp.)