Table 2.

DMAS-RT-PCR findings on cell cultured SARS-CoV-2 isolates.

Viral Isolate	Genotype by sequencing	Reaction ‘A’ for non-Delta	Reaction ‘B’ for Delta	Genotype by DMAS-RT-PCR
IC19	‘wild type’*	Detected at all dilutions⁎⁎	Not detected at any dilution	Not Delta variant
Alpha #246	Alpha variant	Detected at all dilutions⁎⁎	Not detected at any dilution	Not Delta variant
Beta #65	Beta variant	Detected at all dilutions⁎⁎	Not detected at any dilution	Not Delta variant
Beta #78	Beta variant	Detected at all dilutions⁎⁎	Not detected at any dilution	Not Delta variant
Delta #395	Delta variant	Not detected at any dilution⁎⁎	Detected at all dilutions	Delta variant
Delta #02510	Delta variant	Detected at 1:1000 only; 18.8 cycles later than Reaction ‘B’	Detected at all dilutions	Delta variant

*IC19 is a B.1 lineage virus similar to the original ‘wild-type’ Wuhan virus but with the D614G spike mutation.

^⁎⁎Ten-fold dilutions 1:1000, 1:10,000, 1:100,000 and 1:1000,000 were tested for each isolate.