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. Author manuscript; available in PMC: 2021 Nov 29.
Published in final edited form as: Nat Metab. 2021 Mar 15;3(3):352–365. doi: 10.1038/s42255-021-00364-0

Extended Data Figure 8.

Extended Data Figure 8

Generation and characterization of IR1-DTR.

(a) A schematic diagram illustrating knock-in strategy for generation of IR1-DTR by homologous recombination using CRISPR/Cas9. (b) Strategy for labeling pancreatic β cells by Ins2-Dre;IR1-DTR mouse line. (c) Immunostaining for tdT, zsGreen and Ins on pancreatic slides collected from 8 weeks old Ins2-Dre;IR1-DTR. (d) Immunostaining for tdT, zsGreen, and DTR on pancreatic slides collected from 8 weeks old Ins2-Dre;IR1-DTR. (e) Schematic figure showing experimental strategy of DT injection and analysis. (f) Whole-mount bright-field and fluorescent images of pancreas from Ins2-Dre;IR1-DTR mice. (g) Immunostaining for tdT, zsGreen, and Ins on pancreatic sections of Ins2-Dre;IR1-DTR mice. (h) Immunostaining for tdT, zsGreen, and DTR on pancreatic sections of Ins2-Dre;IR1-DTR mice. Scale bars, yellow, 1 mm; white, 100 μm. Each image is representative of 5 individual biological samples.