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(A) Quantitative analysis of peptides recovered in the precipitation pellet and supernatant fractions through reversed-phase LC, coupled with UV absorbance. (B,C) The resulting base peak chromatograms from LC-MS/MS analysis of the fractions demonstrate clear differences between samples. The inset shows the cumulative MS spectra from each sample, suggesting greater complexity in the pellet. The sample comprises a mixture of 5 standard proteins, following digestion with pepsin.
