Table 1.
Normalized frequency of amino acid residues contained on peptides recovered in the pellet and supernatant fraction following precipitation.
| Amino Acid Residues | Average of Normalized 1 Residues to the Length of the Peptides) | p Value of t-Test | |
|---|---|---|---|
| Pellet | Supernatant | Pellet vs. Supernatant |
|
| Basic Residues (K + R + H) | 0.12 ± 0.08 | 0.06 ± 0.07 | 1.46 × 10−34 |
| Histidine (H) | 0.02 ± 0.03 | 0.01 ± 0.02 | 7.84 × 10−22 |
| Lysine (K) | 0.06 ± 0.06 | 0.03 ± 0.05 | 5.96 × 10−18 |
| Arginine (R) | 0.04 ± 0.05 | 0.02 ± 0.04 | 7.31 × 10−15 |
| Leucine (L) | 0.10 ± 0.09 | 0.14 ± 0.11 | 2.40 × 10−15 |
| Phenylalanine (F) | 0.04 ± 0.06 | 0.05 ± 0.07 | 5.32 × 10−3 |
| Cysteine (C) | 0.00 ± 0.02 | 0.00 ± 0.01 | 5.69 × 10−3 |
| Aspartic Acid (D) | 0.05 ± 0.06 | 0.05 ± 0.07 | 2.50 × 10−2 |
| Serine (S) | 0.06 ± 0.07 | 0.05 ± 0.07 | 3.34 × 10−2 |
| Tyrosine (Y) | 0.03 ± 0.05 | 0.04 ± 0.07 | 4.30 × 10−2 |
| Isoleucine (I) | 0.06 ± 0.07 | 0.07 ± 0.09 | 4.52 × 10−2 |
| Acidic Residues (D+E) | 0.11 ± 0.09 | 0.11 ± 0.09 | ND |
| Alanine (A) | 0.09 ± 0.09 | 0.09 ± 0.09 | ND |
| Glutamic Acid (E) | 0.06 ± 0.07 | 0.05 ± 0.07 | ND |
| Tryptophane (W) | 0.01 ± 0.03 | 0.01 ± 0.02 | ND |
| Glutamine (Q) | 0.03 ± 0.05 | 0.03 ± 0.05 | ND |
| Glycine (G) | 0.08 ± 0.08 | 0.07 ± 0.08 | ND |
| Proline (P) | 0.06 ± 0.06 | 0.06 ± 0.07 | ND |
| Valine (V) | 0.08 ± 0.08 | 0.08 ± 0.09 | ND |
| Methionine (M) | 0.01 ± 0.03 | 0.01 ± 0.04 | ND |
| Threonine (T) | 0.05 ± 0.06 | 0.05 ± 0.07 | ND |
| Asparagine (N) | 0.04 ± 0.06 | 0.04 ± 0.06 | ND |
1 Normalize residue numbers are obtained by dividing the given residue count by the respective peptide length.