FIG. 7.
Effect of RhoGN17 on RasV12-induced neurite outgrowth. (A) PC12 cells were cotransfected with an expression vector encoding HA-tagged RasV12 and an empty vector (a and b) or a vector encoding Myc-tagged RhoGN17 (c and d). At 48 h after transfection, cells were fixed and costained with anti-HA (a and c) and anti-Myc (b and d) antibodies. The results shown are representative of three independent experiments. Bar, 25 μm. (B) Quantification of the effect of RhoGN17 on RasV12-induced neurite outgrowth. At 48 h after transfection, cells were costained with anti-Myc and anti-HA antibodies, and positively stained cells were assessed as described in the legend to Fig. 1. GFP-transfected cells were used as a control. Data are the means ± standard errors of triplicate experiments.