FIG. 3.

Deletion of ISS increases exon IIIb inclusion in both DT3 and AT3 cell lines when tested in the absence of exon IIIc. (A) Outline of the constructs used that contain exon IIIb. Designations are the same as in Fig. 2A, except that here a hatched box is added to indicate substitution of the β-globin intron sequence. (B) The boxed figures at the left indicate the two expected products in which either IIIb is included or exon IIIb is skipped and the upstream (U) and downstream (D) adenovirus exons are spliced. Quantifications graphed in the right panel were performed as described in Materials and Methods. (C) Substitution of β-globin intron sequence has the same effect as deletion of ISS.