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Published in final edited form as: Water Res. 2021 Aug 19;205:117571. doi: 10.1016/j.watres.2021.117571

Figure 3. — (A to D) Unrooted neighbor-joining dendrograms of PCR-amplified 16S rRNA gene sequences (obtained from clone libraries) of Legionella for (A) SW1, (B) SW2, (C) SW3, and (D) GW. Test scores based on bootstrap values (1,000 replicates) are shown at the nodes. For the samples from the current study, the name of each branch provides the following information (in this order): Sampling date [December 2017 (i.e., Dec) or March 2018 (i.e., Mar)]; Water utility name (i.e., SW1, SW2, SW3, or GW); Sampling site; and number (n) of Legionella-positive clones/sequences (1 to 12; sequences with a similarity ≥ 99% were clustered into one branch). For the reference species or sequences, the name of each branch provides the following information (in this order): Name of the species or sequence; Place/sample where the species or species was isolated or identified from; and NCBI accession number (in the round brackets). DW: Drinking water. DWDS: Drinking water distribution system. DWST: Drinking water storage tank. NCBI: National Center for Biotechnology Information (ncbi.nlm.nih.gov). AY957925: Closet match in GenBank is Legionella/Amoeba proteus symbiont. CP021286: Originally isolated from human lung tissue. CP025491 and CP045308: Isolated from patients with Legionnaires’ disease. FJ544432: Isolated from ozonated water. KM504126: Isolated from bronchoscopy specimens of cancer patients (bronchoalveolar lavage). LC504038: Originally isolated from lung biopsy. NR_109415: Identical to JF779685. NR_109416: Identical to JF779686. NR_115062: Identical to AM747393. NR_044956: Identical to Z49722 (originally isolated from cooling tower water). Z49719 (current name: Fluoribacter bozemanae): Originally isolated from necropsy lung tissue of a patient with pneumonia. References: AB933973: Inoue et al. (2015). AY957925: Williams et al. (2005). CP021286: Kozak-Muiznieks et al. (2018). CP025491: Slow et al. (2018). FJ544432: Corsaro et al. (2010). KM624123: Lu et al. (2015). KM504126: Han et al. (2015). KP025620: Lu et al. (2016). KX238931 and KX238939: Lu et al. (2017). LC491288: Edagawa et al. (2019). MN865866: Ibrahim et al. (2020). NR_109415 and NR_109416: Campocasso et al. (2012). NR_115062: Lück et al. (2010). NR_044956: Hookey et al. (1996) and Thacker et al. (1991). Z49719: Hookey et al. (1996), Garrity et al. (1980), and Cordes et al. (1979). (E) Shannon’s diversity index values for the 20 sampling events (the four sampling events for GW in December 2017 and all sampling events for the four CDWDSs in March 2018). For each sampling event, the two DNA extracts from the two biological replicates (i.e., the two drinking water samples) were mixed and then used as the template in the PCR.

Figure 3. — (A to D) Unrooted neighbor-joining dendrograms of PCR-amplified 16S rRNA gene sequences (obtained from clone libraries) of Legionella for (A) SW1, (B) SW2, (C) SW3, and (D) GW. Test scores based on bootstrap values (1,000 replicates) are shown at the nodes. For the samples from the current study, the name of each branch provides the following information (in this order): Sampling date [December 2017 (i.e., Dec) or March 2018 (i.e., Mar)]; Water utility name (i.e., SW1, SW2, SW3, or GW); Sampling site; and number (n) of Legionella-positive clones/sequences (1 to 12; sequences with a similarity ≥ 99% were clustered into one branch). For the reference species or sequences, the name of each branch provides the following information (in this order): Name of the species or sequence; Place/sample where the species or species was isolated or identified from; and NCBI accession number (in the round brackets). DW: Drinking water. DWDS: Drinking water distribution system. DWST: Drinking water storage tank. NCBI: National Center for Biotechnology Information (ncbi.nlm.nih.gov). AY957925: Closet match in GenBank is Legionella/Amoeba proteus symbiont. CP021286: Originally isolated from human lung tissue. CP025491 and CP045308: Isolated from patients with Legionnaires’ disease. FJ544432: Isolated from ozonated water. KM504126: Isolated from bronchoscopy specimens of cancer patients (bronchoalveolar lavage). LC504038: Originally isolated from lung biopsy. NR_109415: Identical to JF779685. NR_109416: Identical to JF779686. NR_115062: Identical to AM747393. NR_044956: Identical to Z49722 (originally isolated from cooling tower water). Z49719 (current name: Fluoribacter bozemanae): Originally isolated from necropsy lung tissue of a patient with pneumonia. References: AB933973: Inoue et al. (2015). AY957925: Williams et al. (2005). CP021286: Kozak-Muiznieks et al. (2018). CP025491: Slow et al. (2018). FJ544432: Corsaro et al. (2010). KM624123: Lu et al. (2015). KM504126: Han et al. (2015). KP025620: Lu et al. (2016). KX238931 and KX238939: Lu et al. (2017). LC491288: Edagawa et al. (2019). MN865866: Ibrahim et al. (2020). NR_109415 and NR_109416: Campocasso et al. (2012). NR_115062: Lück et al. (2010). NR_044956: Hookey et al. (1996) and Thacker et al. (1991). Z49719: Hookey et al. (1996), Garrity et al. (1980), and Cordes et al. (1979). (E) Shannon’s diversity index values for the 20 sampling events (the four sampling events for GW in December 2017 and all sampling events for the four CDWDSs in March 2018). For each sampling event, the two DNA extracts from the two biological replicates (i.e., the two drinking water samples) were mixed and then used as the template in the PCR.