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. Author manuscript; available in PMC: 2021 Nov 29.
Published in final edited form as: ACS Chem Neurosci. 2020 Jul 9;11(15):2277–2285. doi: 10.1021/acschemneuro.0c00214

Figure 4.

Figure 4.

Dose–response by fluorescence polarization of eight positive hits with calculated IC50’s. Relative IC50 determined through a variable slope nonlinear regression model using the equation Y = bottom + (top − bottom)/1 + (IC50/X)HillSlope. Line fitting was performed using GraphPad Prism8 analysis tools. N = 3 biological replicates for each compound.