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. 2021 Aug;22(8):2587–2596. doi: 10.31557/APJCP.2021.22.8.2587

Table 1.

Primer Sequences Used in Real-Time PCR Technique

Genes Primer Sequence PCR product size (bp)
hTERT F: 5′-CCCATTTCATCAGCAAGTTTGG-3′ 94
R: 5′-CTTGGCTTTCAGGATGGAGTAG-3′
GAPDH F: 5′-CATGAGAAGTATGACAACAGCCT-3′ 113
R: 5′-AG TCCTTCCACGATACCAAAGT-3′
bcl-2 F: 5′ GATGTGATGCCTCTGCGAAG -3′ 93
R: 5′-CATGCTGATGTCTCTGGAATCT-3′
Bax F: 5′-GGTTGTCGCCCTTTTCTA-3′ 98
R: 5′- CGGAGGAAGTCCAATGTC -3′
caspase-3 F: 5′- TTCAGAGGGGATCGTTGTAGAAGTC -3′ 178
R: 5′- CAAGCTTGTCGGCATACTGTTTCAG -3′
caspase-7 F: 5′-GGACCGAGTGCCCACTTATC-3′ 149
R: 5′-TCGCTTTGTCGAAGTTCTTGTT-3′
Cyclin D1 F: 5′-AGACCTTCGTTGCCCTCTGT-3′ 181
R: 5′-CAGTCCGGGTCACACTTGAT-3′
Survivin F: 5′ GATGTGATGCCTCTGCGAAG -3′ 103
R: 5′-CATGCTGATGTCTCTGGAATCT-3′
P 53 F: 5′- GACGGTGACACGCTTCCCTGGATT -3′ 120
R: 5′- GGGAACAAGAAGTGGAGAATGTCA -3′