Fig. 4.

Interaction of membrane proteins PGRMC1 and PGRMC2 with DAT. HEK293 cells were transfected with pCDNA3 or HA-DAT plus myc-PGRMC1 or myc-PGRMC2 in the combinations indicated in the different panels. 48 h later, cells were lysed with a modified RIPA buffer (either without SDS or containing the indicated amount of SDS, in %), and the extracts were immunoprecipitated with anti-HA (IP: αHA). Both the IP material (left panels) and samples of the lysates (right panels) were analyzed by western blotting. Immunoblots were probed with anti-HA (WB: αGFP) or anti-myc (WB: αHA) to visualize the different tagged proteins