Fig. 11.

Senescence of ouabain-resistant END-MSCs is accompanied by acquisition of apoptosis-resistant phenotype, while ouabain-sensitive cells become apoptosis-prone during senescence. a GSEA results for the DEGs between the cells resistant to ouabain-mediated senolysis (END-MSCs) and the ones sensitive to ouabain-mediated senolysis (A549 and IMR-90) in the apoptosis-related terms. b Heatmap reflecting expression of the core enrichment genes for apoptosis-related terms from a. The common pro-apoptotic genes are marked with asterisks. c, d Relative cell viability (%) of control and senescent END-MSCs or A549 treated either with H₂O₂ or staurosporine at indicated concentrations. e Growth curves of control and senescent END-MSCs treated with A-1210477. f Relative cell viability (%) of control and senescent END-MSCs pretreated with A-1210477 followed by ouabain or bufalin supplementation. Red line marks the initial percent of viable cells (prior to treatment) normalized to 100% both for the control and senescent ESCs. Values are mean ± SD. Statistical significance was assessed by the two-tailed Student's t test: *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant