Fig. 6.

Validation of etoposide-induced A549 senescence model. Senescent A549 cells display a loss of proliferation, b acquire elevated cell size, c autofluorescence level and d SA-b-Gal activity compared to the control ones. e Phosphorylation levels of p53 and Rb and expression levels of p21 and HMGB1 proteins in control and senescent A549. Values presented are mean ± SD. For multiple groups comparisons at a one-way ANOVA was applied, n = 3, ns not significant, ***p < 0.001. For pair comparisons at b, c and d Welch’s t test was used, n = 3 for b and c, n = 50 for d, ***p < 0.001. Scale bars for images are 500 μm. GAPDH was used as loading control