Fig. 7.

Cardiac glycosides induce cell death in etoposide-treated senescent A549 cells. a, b Relative cell viability (%) of control and senescent A549 in 3 days after treatment with 10^–7, 10^–6, 10^–5 M ouabain/bufalin. c Apoptosis induction in A549 upon 10^–6 M ouabain assessed by Annexin V/DAPI double staining. d Activity of Caspase-3/7 estimated in control and senescent A549 treated with 10^–6 M ouabain. n = 3 independent experiments. All data correspond to the mean ± SD. Statistical significance was assessed by the Welch’s t test: ns not significant, *p < 0.05, **p < 0.01