FIG. 1.

Targeting the FHL2 gene. (a) Restriction maps of the FHL2 genomic region of interest (top), the targeting construct (center), and the mutated locus after homologous recombination (bottom). A, ApoI; B, BamHI; Bg, BglII; C, ClaI; Cv, CvnI; E, EcoRI; K, KpnI; N, NotI; S, SstI; X, XbaI. (b) Detection of the heterozygous recombinant FHL2 allele by Southern blot analysis. DNAs from neo-positive ES cells were digested with SstI and analyzed by Southern blotting with the probe shown in the top line of panel a. (c) Detection of FHL2 mRNA by Northern blot analysis. Aliquots of 10 μg of total RNA isolated from adult ventricles of wild-type (+/+) and FHL2-deficient (−/−) mice were analyzed by using a cDNA probe spanning the entire FHL2 coding region. EF-1α cDNA was used as a control probe to normalize for loading.