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. Author manuscript; available in PMC: 2022 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2021 Oct 26;714:109064. doi: 10.1016/j.abb.2021.109064

Figure 4. The dilution experiment expands the experimental concentration range.

Figure 4.

The dilution factor for all dilution simulations is equal to 10. Extinction coefficients for the bound species are equal to 1. IF: (Rapid binding example from Figure 2A) kon = 100 mM−1 s−1, koff = 50 s−1, kf = 1 s−1, kr = 0.8 s−1, KD,app = 0.22 mM, KD = 0.5 mM, CS: (Rapid binding example from Figure 2D) kf = 1 s−1, kr = 0.8 s−1, kon = 100 mM−1 s−1, koff = 50 s−1, KD,app = 0.9 mM, KD = 0.5 mM.