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. 2021 Nov 29;11:23060. doi: 10.1038/s41598-021-02428-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogeographic analysis of the evolution of HIV-1 subtype C transmission clusters. (A) Bayesian MCC time scaled discrete phylogeographic tree built using BEAST v1.10.4 of the HIV-1 subtype C sequences included in the transmission clusters that were sampled in Brazil and have complete information (n = 156). Tip location was defined as Brazilian region or state of sample collection. (B) Geographical representation of this transmission history. Acronyms and number of sequencies per location: N (North region of Brazil, n = 11), NE (North-East region of Brazil, n = 4), CW (Central-West region of Brazil, n = 14), SE (South-East region of Brazil, n = 34), SC (state of Santa Catarina in the South region of Brazil, n = 43), RS (state of Rio Grande do Sul in the South region of Brazil, n = 31), and PR (state of Paraná in the South region of Brazil, n = 19). SpreaD3 v0.9.6 (https://rega.kuleuven.be/cev/ecv/software/SpreaD3) was used to visualize the phylodynamic reconstruction resulting from Bayesian inference.