Skip to main content
. 2021 Nov 29;12(12):1116. doi: 10.1038/s41419-021-04406-z

Fig. 5. LncFERO promotes SCD1 expression by recruiting hnRNPA1 in GCSCs.

Fig. 5

A Levels of lncFERO in exosomes isolated from GC cells treated with lncFERO-OE plasmids or siRNAs (n = 3). B Effects of GC-secreted lncFERO on SCD1 mRNA levels in GCSCs (n = 3). C Effects of GC-secreted lncFERO on SCD1 expression in GCSCs (n = 3). D lncFERO in GCSCs was overexpressed or knocked down (n = 3). E Effects of lncFERO on the expression of SCD1 mRNA in GCSCs (n = 3). F Western blotting analysis of SCD1 expression in GCSCs with overexpression or knockdown of lncFERO (n = 3). G RBPDB analysis of the specific interaction between lncFERO and RBP motifs. H The colocalization of Cy3-lncFERO and hnRNPA1 in GCSCs (n = 3). I RNA immunoprecipitation assay shows the direct interaction between lncFERO and hnRNPA1 in GCSCs (n = 3). J Detection of hnRNPA1 protein in the samples derived from lncFERO pulldowns performed in GCSCs. K GC exosomal lncFERO decreased PUFA levels in GCSCs (n = 3). L GC exosomal lncFERO promoted the stemness of GCSCs (n = 3). **p < 0.01 and ***p < 0.001.