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. 2021 Jun 15;28(12):3251–3269. doi: 10.1038/s41418-021-00813-z

Fig. 2. ATG9B promotes CRC cell invasion and lung metastasis.

Fig. 2

A, B Boyden chamber invasion assay showing the invasion ability of ATG9B upregulation (A) and downregulation (B) in the indicated cells. Quantification of invaded cells are shown in the right panel (scale bar 50 μm, n = 5). C Representative H&E staining images of lung metastasis in mice injected with SW480/Vector, SW480/ATG9B, DLD1/Vector or DLD1/ATG9B cells. The black boxes represents the magnified sites (scale bar 20 μm, n = 6). D Quantification of the lung weights from the experiment like in C (n = 6). E Quantification of metastasis numbers from the experiment like in C (n = 6). F Representative H&E staining images of lungs metastasis in xenograft-bearing mice with SW620/shCtrl, SW620/shATG9B, LoVo/shCtrl or Lovo/shATG9B cells. The black boxes represents the magnified sites (scale bar 20 μm, n = 6). G Quantification of the lung weights from the experiment like in F (n = 6). H Quantification of metastasis numbers from the experiment like in F (n = 6). I–L Kaplan–Meier survival analysis of xenograft-bearing mice with SW480/Vector or SW480/ATG9B cells (I), DLD1/Vector or DLD1/ATG9B cells (J), SW620/shCtrl or SW620/shATG9B cells (K), LoVo/shCtrl or Lovo/shATG9B cells (L). Data information: Graphs report mean ± SEM. Significance was assessed using two-tailed Student’s t test, except for I–L where log-rank test was used. ***P < 0.001, **P < 0.01, *P < 0.05.