Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 15;28(12):3251–3269. doi: 10.1038/s41418-021-00813-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Double IF staining detected the distribution of exogenous ATG9B (yellow colour) in SW480 cells transfected with vector, ATG9B^His or ΔATG9B^His (deleted mutation with aa368–411 of ATG9B). The magnified parts are displayed in lower panel, scale bar 10 μm. B IF staining to detect pY397-FAK (red) expression in the indicated cells. The magnified parts are displayed in lower panel, scale bar 10 μm. C Immunofluorescence images of pY118-Paxiilin in SW480/ATG9B^EGFP cells after treatment with 10 μM nocodazole (NZ) for 4 h followed by washout for the indicated times, scale bar 10 μm. The magnified parts of vector cells (dotted line) and ATG9B^EGFP overexpression cells (solid line) were, respectively, displayed. Mean intensity levels of surface pY397-Paxillin at the indicated times of NZ washout is presented in the right panel (n = 20 cells analysed per group). D Time-limited FN adhesion assay to detect the rate of FA formation in the indicated cells for 30 min. Scale bar 50 μm (n = 5). Data information: Graphs report mean ± SEM. Significance was assessed using two-tailed Student’s t test. ***P < 0.001, **P < 0.01, NS, no significance.