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. 2021 Nov 29;12:6964. doi: 10.1038/s41467-021-27286-5

Fig. 1. PET fluorescence reporter design and immobilization of Hsp90.

Fig. 1

a Left: Structural model of apo Hsp90 based on crystallographic data of the NTD (pdb id 1AM1) and the MC-domain (pdb id 2CGE). NTD (N), charged linker (CL), MD (M), and CTD (C) are indicated. The ATP-binding pocket is indicated by a black arrow. Right: Crystal structure of Hsp90 in closed-clamp conformation with bound AMP-PNP (pdb id 2CG9). Mutations and fluorescence modifications (coloured spheres) constituting the PET reporters for Lid (green), NM-association (orange) and DS (magenta) are highlighted. Conformational changes initiated by binding of nucleotide and probed by PET reporters are indicated by coloured arrows. b Scheme of sm immobilization of hetero-dimeric Hsp90. A PET fluorescence reporter for NM-association is indicated (orange arrow).