Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 29;12:6964. doi: 10.1038/s41467-021-27286-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Relative number of synchronous transitions detected from two-colour smPET fluorescence intensity time traces recorded from pairwise reporters for Lid, DS and NM-association. b–e Cumulative sum plots of dwell times in fluorescent states (kinetics of closure) and in fluorescence-quenched states (kinetics of opening) determined from the reporter pairs Lid^Atto542-NM^JF646 (b), DS^Atto542-NM^JF646 (c), DS^Atto542-Lid^JF646 (d) and Lid^Atto542-A2W-NM^JF646 (e). Data shown in green and magenta are from fluorescence of Atto542 and JF646, respectively. Black lines are mono-exponential fits to the data. Cyan lines are bi-exponential fits to the data. f Mean rate constants of closure (closed bars) and opening (open bars) determined from fits to the data shown in b–e. Values are the mean of three measurements and errors are standard deviations (n = 3; ±s.d.). g Bulk fluorescence measurements of kinetics of NM-association of Hsp90 probed by a one-colour PET reporter involving JF646 as a probe (orange). Data from the same construct but containing mutation A2W on the opposite subunit are shown in blue. Measurements were carried out on a benchtop fluorimeter. 4 mM AMP-PNP was added at t = 0 min. Cyan lines are bi-exponential fits to the data. Source data are provided as a Source Data file.