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. 2021 Nov 29;12:6963. doi: 10.1038/s41467-021-27161-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Immunofluorescence (IF) staining for endothelial markers of Stab2-iCreF3;R26YFP bone marrow from 6-month-old female mice. Co-IF of YFP, DAPI and a EMCN (n = 3), b Stab2 (n = 3), c CD32b, α-SMA (n = 3), d VEGFR3, and e CAV1. Scale bars: 50 μm. f–h Hematopoietic marker staining of Stab2-iCreF3;R26YFP bone marrow from 5-month-old female mice for IF and flow cytometry analysis. f Co-IF of YFP, DAPI, CD45 (n = 3). Scale bars: 50 μm. Representative graphs of flow cytometry YFP quantification in g Lin^-Sca-1⁺Kit⁺, Lin^-Sca-1^-Kit⁺ cells, T cells (CD3⁺), B cells (CD19⁺), myeloid cells (Gr1^-CD11b⁺, Gr1⁺CD11b⁺) of g bone marrow and h spleen (n = 4). Source data for g, h are provided as a Source Data file.