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. 2021 Nov 29;12:6963. doi: 10.1038/s41467-021-27161-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Kaplan–Meier survival curves for control (Ctnnb1^WT) and Ctnnb1^OE-SEC mice. The probability of survival is shown for Ctnnb1^OE-SEC female (n = 18) vs. Ctnnb1^OE-SEC male (n = 30) vs. Ctnnb1^WT mice (n = 25). b–f Red blood cell indices for 2-, 3-, and 5-month-old male Ctnnb1^WT and Ctnnb1^OE-SEC mice. b Red blood cells (RBC) (2 months, n ≥ 3; 3 months, n = 4; 5 months, n ≥ 7), c hemoglobin (Hb) (2 months, n ≥ 3; 3 months, n = 9; 5 months, n ≥ 7), d hematocrit (HCT) (2 months, n ≥ 3; 3 months, n = 4; 5 months, n ≥ 7), e mean corpuscular volume (MCV) (2 months, n ≥ 3; 3 months, n = 4; 5 months, n = 8), f mean corpuscular hemoglobin (MCH) (2 months, n ≥ 3; 3 months, n = 4; 5 months, n = 8). g Platelet (3 months, n = 4; 5 months, n = 8) and h white blood cell (WBC) counts (3 months, n = 4; 5 months, n = 8) for 3- and 5-month-old male Ctnnb1^WT and Ctnnb1^OE-SEC mice. i Peripheral blood smears of 2- and 3-month-old female and 5-month-old male Ctnnb1^WT and Ctnnb1^OE-SEC (n ≥ 3) mice. Scale bars: 100 μm. j H&E staining of bone marrow sections of 3-month-old female Ctnnb1^WT and Ctnnb1^OE-SEC (n = 5). Scale bar: 100 μm. k Bone marrow smears of 5-month-old male Ctnnb1^WT and Ctnnb1^OE-SEC (n = 3) mice (×100 enlargement). l, m Representative transmission electron microscopy analysis of Ctnnb1^WT and Ctnnb1^OE-SEC bone marrow. l Arrows indicate erythroblasts, reticulocytes in the bone marrow parenchyma (×1250 enlargement). m Arrows indicate reticulocytes outside of sinusoidal walls (×5000 enlargement). 3- and 4-month-old female Ctnnb1^WT and Ctnnb1^OE-SEC mice (n = 3). n, o Reticulocyte analysis in the peripheral blood. n Relative (%), absolute (×10⁹/L) reticulocyte numbers, and immature reticulocyte fraction (IRF) in the peripheral blood of 3- and 5-month-old female Ctnnb1^WT and Ctnnb1^OE-SEC mice (n ≥ 5). o Brilliant cresyl blue staining of blood smears of 5-month-old male Ctnnb1^WT and Ctnnb1^OE-SEC mice (n = 3). Scale bar: 100 μm. The dots in the graphs represent individual mice. Mean ± s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Student’s t-test or Mann–Whitney U test (two-sided). ns, not significant. Source data, precise values of n, and employed statistical tests for a, b, c, d, e, f, g, h, n are provided as a Source Data file.