Fig. 4. BM-SEC in Ctnnb1^OE-SEC mice exhibit aberrant sinusoidal differentiation including increased extracellular matrix (ECM) deposition.

a–f IF staining for endothelial, erythroid and ECM markers of Ctnnb1^WT and Ctnnb1^OE-SEC bone marrow sections of 2-, 3-, or 5-month-old female mice. Co-IF of DAPI, a CD31 and EMCN (n = 3); b Stab2, CD32b (n = 3); c EMCN, Collagen I (Col I) (n = 3); d EMCN, Collagen III (Col III) (n = 3); e EMCN, Collagen IV (Col IV) (n = 3); and f Ter119, Fibronectin (n = 3). Scale bars: 50 μm. Quantifications of IFs are shown next to corresponding IF staining (a, c–f). The dots in the graphs represent individual mice. Three representative images have been used for quantification per individual mouse. Mean ± s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Student’s t-test or Mann–Whitney U test (two-sided). ns, not significant. Source data, and employed statistical tests for a, c, d, e, f are provided as a Source Data file.