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. 2021 Nov 29;12(12):1115. doi: 10.1038/s41419-021-04400-5

Fig. 7. SIRT1 plays a critical role in the effect of MICU3.

Fig. 7

A Correlation graphs between the mRNA expression of MICU3 and SIRT1. B IHC and western blot for SIRT1 in mice from different age groups (magnification ×400; scale bar = 20 μm, *p < 0.05 vs. the 6M group; n = 3). C, D Western blot and quantification of MICU3. VDAC was used as the loading control. *indicates target bands. E Western blot and quantification of SIRT1, PGC1α, Nrf-2, cleaved caspase-3, cleaved caspase-9, Procaspase-3, and Procaspase-9. GAPDH was used as the loading control. (*p < 0.05 vs. the Ad-EC group; #p < 0.05 vs. the Ad-EC + d-gal group; &p < 0.05 vs. the Ad-MICU3 + d-gal group; n = 3). F Western blot and quantification of MICU3. VDAC was used as the loading control. *indicates target bands. G Western blot and quantification of SIRT1, PGC1α, Nrf-2, cleaved caspase-3, cleaved caspase-9, Procaspase-3, and Procaspase-9. GAPDH was used as the loading control. (*p < 0.05 vs. the si-NC group; #p < 0.05 vs. the si- MICU3 group; &p < 0.05 vs. the si-NC + SRT2104 group; n = 3). Data were expressed as means ± SEM, and data were analyzed using a one-way ANOVA.