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. 2021 Nov 29;12:6967. doi: 10.1038/s41467-021-27220-9

Fig. 6. Compressive stress and EZH2-dependent histone 3 trimethylation regulate ERα expression.

Fig. 6

a Enrichment of the gene-repressive histone trimethylation (H3K27me3) signatures in different MMECs, PDEC-N, and PDEC-BC samples. Different comparisons and the resulting enrichments are shown below of the graphs. b Immunofluorescent staining of ERα expression in control and enhancer of zeste homolog 2 (EZH2) inhibitor GSK-126 treated explants from DU4475 cells, MMECs, PDEC-N, and PDEC-BC. N= 6 explants examined from three biologically independent samples. c PDEC-BCs from four patients (PxxxT) were exposed to anisomycin or GSK-126 and the effect on ERα-regulated GREB1 and PGR genes was measured using QRT-PCR. d Western blot analysis shows ERα, CK5 and H3K27me3 expression in the DU4475 cells after 48 h treatment with anisomycin (n= 3). e A model for the stress-mediated regulation of ERα expression. f Illustration of the magnetic cylinder-mediated compression method. g Immunofluorescence images of PDEC-Ns and PDEC-BCs in the LMx-Ag following overnight compression with the magnetic cylinders, stained as indicated. N = 4 explants examined from 3 biologically independent samples. h PDEC-BCs from three patients were exposed to magnet mediated compression for 48 h and with or without tamoxifen treatment. The effect on ERα regulated GREB1 and PGR genes was measured with QRT-PCR. Scale bar = 10 μm.