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. 2021 Nov 29;12:6967. doi: 10.1038/s41467-021-27220-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Immunofluorescent staining of p38p, ERα, and H3K27me3 expression in LMx-Ag cultured MMECs after p38 (RWJ67657) and JNK (SP600125) inhibition. N = 4 explants examined from five biologically independent samples. b Western blot images of ERα, EZH2 p(T367), and H3K27me3 expression in LMx-Ag cultured MMECs after p38 (RWJ67657) and JNK (SP600125) inhibition (n = 3). c Immunofluorescent staining of p38p, ERα, and H3K27me3 expression in magnetic compressed LMx-Ag cultured PDEC-BCs after p38 (RWJ67657) and JNK (SP600125) inhibition. N= 4 explants examined from five biologically independent samples. d Western blot images of EZH2 p(T367), H3K27me3, and ERα (with and without magnet) expression in magnet compressed LMx-Ag cultured PDEC-BC after p38 (RWJ67657) and JNK (SP600125) inhibition (n= 3). e Immunofluorescent images of magnet compressed PDEC-BCs treated with and without p38 inhibitor (RWJ67657) and EZH2 inhibitor (GSK-126). Expression of ERα is shown in green and F-actin is shown in red. N= 4 explants examined from five biologically independent samples. f Immunohistological staining of phosphorylated p38 and ERα in reduction mammoplasty (RMP) and breast cancer samples (PxxxT). The pie chart shows the coincidence of ERα and phospho-p38 expression in breast cancer samples (n = 42). Sequential sections were stained. The statistical significance was calculated between two cohorts (a: n = 17 and b: n = 25) using 2-sided Pearson’s correlation test, indicating that the values in the four classifications are linearly correlated between the two data sets. g, Correlation between ERα+ positivity and the breast mammographic density (MGR) in reduction mammoplasty samples. Grade 1: samples with 10–24% of ERα positive cells; Grade 2. Samples with 25–49% of ERα+ cells; Grade 3. Samples with 50–74% of ERα+ cells; Grade 4. Samples with 75–100% of ERα+ cells. N = 18 from biologically independent samples. h Model figure summarizing the main findings. Scale bar 10 μm.