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. 2021 Jun 26;28(12):3316–3328. doi: 10.1038/s41418-021-00824-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Relative mRNA expression levels of a panel of key transcription factors potentially regulating p21 normalized to GAPDH analyzed by quantitative real-time PCR in scrambled control (Scr) or EZH2 knockdown ISK and KLE cells. Data shown are mean ± SD (n = 3). **P < 0.01 compared with Scr control. B Immunoblots of EZH2 and TCF3 in Scr or PRC2 core subunit (EZH2, EED, and SUZ12) knockdown ISK and KLE cells. GAPDH served as a loading control. C Enrichment of H3K27me3 on TCF3 promoter in Scr or EZH2 knockdown ISK cells detected by ChIP-qPCR (bottom). Diagram shows positions of ChIP primers at the TCF3 promoter (top). Data shown are mean ± SD (n = 3). **P < 0.01, *P < 0.05 compared with indicated control. D Immunoblots of TCF3, p21, and EZH2 in negative control (NC, vector alone) or enforced expression of TCF3 variants (E12 or E47) in ISK and KLE cells. GAPDH served as a loading control. E, F Cellular proliferation were measured by CCK8 (E) and colony formation assay (F) after enforced expression TCF3 variants (E12 or E47) in ISK and KLE cells. **P < 0.01 vs. NC. G Effect of inhibition of EZH2 activity by GSK126 and EPZ-6438 on TCF3 and p21 protein levels in ISK (left) and KLE (right) cells. GAPDH served as a loading control. H Cell proliferation measured by CCK8 assay after GSK126 and EPZ-6438 treatment in ISK and KLE cells. **P < 0.01 vs. Vehicle treatment. I Immunohistochemical (IHC) staining of TCF3 protein in matched human normal and EC tissues (left). Representative micrographs are shown in original magnification (×400). Scale bars, 50 μm. Total IHC score of TCF3 in matched human normal and EC tissues (n = 26). ***P < 0.001 vs. Normal control (right).