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. 2021 Jun 26;28(12):3316–3328. doi: 10.1038/s41418-021-00824-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Immunoblots of EZH2, TCF3, or p21 following knocking down of EZH2 or TCF3 individually or simultaneously in ISK (left) and KLE (right) cells. GAPDH was used as internal loading control. B, C Cellular proliferation measured by CCK8 (B) and colony formation (C) assays following knockdown of EZH2 or TCF3 individually or simultaneously in ISK and KLE cells (as indicated). **P < 0.01 vs. indicated control. D–F Photograph (D), tumor volumes (E), and tumor masses (F) of excised xenograft tumors from scrambled (Scr) control, EZH2-KD, TCF3-KD, and EZH2-KD + TCF3-KD groups. Data are presented as the means ± SD; ***P < 0.001, *P < 0.05 vs. indicated control. G Western blotting analysis of indicated proteins in excised xenograft tumors from Scr control, EZH2-KD, TCF3-KD, and EZH2-KD + TCF3-KD groups. GAPDH and histone H3 were used as endogenous loading controls. H Pearson correlation scatter plot of H scores (protein levels) of EZH2 and TCF3 (left) or p21 (middle), or H scores (protein levels) of TCF3 and p21 (right) in human EC (n = 26).