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. 2021 Nov 29;11:23101. doi: 10.1038/s41598-021-02346-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EGCG3”Me upregulated TLR5 expression on splenocytes. (A–C) Female BALB/c mice were sacrificed and the spleens were harvested. Splenocytes were seeded at 2 × 10⁶ cells/mL in 10% FCS-RPMI-1640 medium in 12-well plates and treated with different doses of EGCG3”Me for 24 h. After treatment, cells were harvested and the mRNA expression was evaluated by RT-qPCR. (A) TLR5 (n = 4), (B) IL-6 (n = 4), (C) IFN-beta (n = 4). Data are presented as means ± SEM ^*P < 0.05 (vs control).