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. 2021 Nov 29;11:23101. doi: 10.1038/s41598-021-02346-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EGCG3”Me intake enhanced TLR5 expression on LPDCs and macrophages of split-virus vaccine-treated mice. (A–E) 12-week-old female BALB/c mice were fed AIN-93G diet or AIN-93G diet supplemented with 0.01% EGCG3”Me (EGCG3”Me 20 mg/kg body weight) for 5 weeks. Mice were treated with split-virus vaccine (1 μg HA/mouse i.d.) at 2 and 4 weeks and then sacrificed at 5 weeks. (A) CD11c⁺CD11b⁺ cells (B, C) and TLR5 expression on CD11c⁺CD11b⁺ cells were determined by flow cytometry (n = 6–8). Data are presented as means ± SEM *P < 0.05.