Skip to main content
. 2021 Nov 29;12:6673. doi: 10.1038/s41467-021-26909-1

Fig. 2. Expression of Ang in inflammatory mice and metabolic parameters of F1 males from inflammatory and Ang-deleted mice.

Fig. 2

a The relative expression level of Ang mRNA in mouse testis, caput epididymis, and cauda epididymis under LPS (Inf) or saline (Con) treatment for 24 h. b Western blots of Ang protein in mouse caput epididymis under LPS or saline treatment for 24 h or 7 d. In (a, b), n = 6 mice per group. c The Ang+/+ and Ang−/− F0 male mice were injected with LPS or saline once every other day for a total of four injections in 7 d in order to establish an inflammatory model. These mice were then allowed to mate with normal females for two days. Subsequently, offspring mice were exposed to chow diet and water ad libitum without any challenge throughout their lives. d Body weight of F1 males at 16 weeks of age. e Ratio of fat-to-muscle weight of F1 males at 16 weeks of age. f Blood glucose levels as assayed by GTT of F1 male mice at 14 weeks of age. ** P < 0.01, *** P < 0.001 (Ang+/+ Inf versus Ang+/+ Con); &P < 0.05, &&P < 0.01, &&&& P < 0.0001 (Ang+/+ Inf versus Ang−/−Con); # P < 0.05, ### P < 0.001 (Ang+/+ Inf versus Ang–/– Inf). g Area under the curve (AUC) statistics for (f). In (f, g), n = 7 mice per group. h Treadmill test results of F1 males at 8 weeks of age. In (d, e) and h, n = 12 in Ang+/+ Con group, n = 13 in Ang+/+ Inf group, n = 10 in Ang–/– Con group, and n = 13 in Ang–/– Inf group. Statistical analysis was performed by two-tailed unpaired Student’s t test (a), by two-tailed, one-way ANOVA (d, e, g, h) or two-way ANOVA (f), uncorrected Fisher’s LSD. Data are show as mean ± SEM. Source data are provided as a Source Data file.