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. 2021 Nov 29;11:23031. doi: 10.1038/s41598-021-02456-z

Figure 1.

Figure 1

MS2 ion spectra of methylated and unmethylated C-terminal peptides for PP2Acα/β, PP4c, and PP6.c. (A) Alignment of the last eight amino acids of the catalytic subunits of the PP2A, PP4, and PP6. (B) Phosphatase inhibitor bead (PIB) pulldown workflow. Cells were lysed. lysates and incubated with PIBs, elutes were digested overnight with either trypsin or LysC and analyzed using LC–MS/MS. (C-E) MS2 ion spectra of the C-terminal peptides for PP2Acα/β, B, PP4c, and PP6c. The unmethylated peptides are shown on the top half of each plot, and the methylated peptide is inverted on the bottom. Ions corresponding to the indicated peptide are shown in black, others are shown in grey.